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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and methods
    • Study area
    • Water, microbe, and stygofauna collections
    • Metazoan sorting and identification
    • Aqueous geochemistry
    • Fluorescence spectroscopy
    • Stable isotope ratio analysis
    • DNA extractions, 16S rRNA gene sequencing, and microbial diversity analysis
    • Biomass calculations based on quantitative PCR
    • Analysis of environmental gradients and stygobiont community composition
    • FPOM source isotope modeling
  • Results
    • Aqueous geochemistry and CDOM characterization
    • Spatial variation of stygobiontic communities
    • Metazoan stable isotope ratios
    • Microbial communities, biomass, and metabolic potential
    • FPOM isotope mixing models based on FAPROTAX metabolic assignments
  • Discussion
    • Distribution of stygofauna
    • Chemolithoautotrophic signals and contributions to stygofaunal diets
    • Hydrological impact on the role of chemolithoautotrophy in the Barton Springs segment
  • Acknowledgements
  • References
  • Additional information
    • Conflict of interest
    • Ethical statement
    • Artificial Intelligence (AI) use
    • Funding
    • Author contributions
    • Author ORCIDs
    • Data availability
  • Supplementary materials
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