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  • ContentsContents
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  • Article title
  • Abstract
  • Keywords
  • Introduction
  • Materials and methods
    • Specimens
    • Molecular methods
    • Molecular tree estimation
    • Divergence time estimation
  • Results
    • Phylogenetic relationships and molecular clock estimates of diversification in the hirtus-group based on mitochondrial DNA sequences
    • Ptomaphagus hatchi is polyphyletic
    • Intraspecific molecular variation
  • Discussion
    • The hirtus-group diversified in three stages
    • How many times have Ptomaphagus invaded cave habitats
    • A new model of hirtus-group diversification: Vicariance by erosion
  • Questions arising from the present study
    • Understanding the lack of hirtus-group species diversity in the Mammoth Cave region
    • Molecular evidence that Ptomaphagus hatchi is polyphyletic
    • “Vicariance by erosion” and the development of a cave biodiversity hotspot on the southern Cumberland Plateau
  • Acknowledgments
  • References
  • Supplementary materials
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