An important site for conservation of bats in Brazil : Passa Três cave , São Domingos karst area , with an updated checklist for Distrito Federal ( DF ) and Goiás state

A checklist of bats from Distrito Federal (DF) and Goiás state (GO) and, particularly a single cave (Passa Três cave), located in São Domingos karst area (GO), central Brazil, is presented. Data is based on literature and surveys carried out during 2,000 years. In total, 66 species were recorded, with 30 using caves as shelters. Passa Três cave harbors nine Phyllostomidae species; the most abundant species were Platyrrhinus lineatus, Lonchorhina aurita, Desmodus rotundus and Carollia perspicillata; and the less abundants Trachops cirrhosus, Anoura caudifer and Glossophaga soricina. Besides, the cave is shelter of two threatened bats Lonchophylla dekeyseri (Endangered category) and Lonchorhina aurita (Vulnerable category), included at Brazilian List of Threatened Fauna, and of a rare species, Lionycteris spurrelli. Passa Três cave shows enough attributes to be considered as a SICOM (Sites of Importance for Conservation of Bats), which would ensure its protection.


Introduction
The Order Chiroptera comprises about 22% of all extant mammals, with more than 1,300 described species (BCI 2018) and currently with 182 species known from Brazil (SBEQ 2018).This number is still increasing, with approximately two new bat species described per year in Brazil in the past two decades (Paglia et al. 2012, Nogueira et al. 2014).In the Cerrado, bats surpass rodents in diversity, with 103 species from all nine families that occur in Brazil (Aguiar and Zortéa 2008), representing more than 50% of all mammal species in this phytophysiognomy.The Cerrado is one of the richest tropical savannas, with a high endemism of plants and birds and a high diversity of vertebrates, being considered a "hotspot" for biodiversity conservation (Mittermeier et al. 1999, Myers et al. 2000).According to Reis et al. (2011), 9% of 195 mammals recorded in the Cerrado are endemic to it.Among bats, the nectarivorous Lonchophylla bokermanni Sazima, Vizotto & Taddei, 1978, was reported as endemic from this hotspot (Nogueira et al. 2014).
The main treats to Cerrado are land expansion for cattle raching, soybean plantations and other land uses that had reduced it to only 20% of the original area (Myers et al. 2000, Strassburg et al. 2017).This can directly affect bat assemblages, changing the habitat structure and reducing shelter and food availability.
Bats use many types of roosts, including caves, rocky crevices, foliage roosts, hollow trees and man-made structures such as the buildings, mines and tunnels (Kunz 1982, Pacheco et al. 2010).For several species, caves are the main roosts (Kunz 1982, Arita 1996, Trajano 1995).Bats are the typical trogloxenes, or organisms that regularly use subterranean habitats but that must return periodically to the surface (in their case, daily to feed) in order to complete their life cycle, and some are obligatory trogloxenes, that depend on caves as obligatory shelters at least during part of their life cycles (Trajano 2012;Trajano and Carvalho 2017).Therefore the conservation of caves and their surrounding are crucial for maintenance of bat populations (Trajano 1995, Arnone 2008, Rocha and Bichuette 2016).
The Red Latinoamericana y del Caribe para la Conservación de los Murciélagos -RELCOM is an international NGO that congregates Latin-American and Caribbean national programs for bat conservation in 22 countries.Among its actions, RELCOM certificates Areas and Sites of Importance for Conservation of Bats (AICOMs and SICOMs), recognized with basis on criteria of diversity, presence of endangered and rare species, maternity colonies, and importance as roosting and migration areas.AICOMs and SICOMs differ only in area size, the latter are smaller.So far, RELCOM certified 80 (64 AICOMs and 16 SICOMs) in 15 countries.However, only recently Brazil have recognized the first and so far the only Brazilian AICOM, the "Alto Ribeira e Alto Paranapanema AICOM", in southeast São Paulo state, SE Brazil (RELCOM 2017).Although many other Brazilian areas are known to fit the RELCOM criteria for Important Areas and Sites for Bat Conservation, no actions have been undertaken to certificate them.
We present herein a checklist of bat species from Distrito Federal and Goiás state, with data of a short-term study in Passa Três cave, São Domingos karst area, central Brazil and part of a State Park (Terra Ronca State Park -PETeR).We discuss the criteria of proposition of SICOMs and the possibility of application to Passa Três cave.
Brazil has ca. of 17,000 recorded caves (CECAV 2018) and a potential to 100,000 according to Auler et al. (2001), and most of this heritage is under threat by mining and other huge economic purposes, such as hydro electrical projects.The São Domingos karst area, Goiás state, central Brazil, is characterized by the presence of huge cave systems crossed by allochthonous streams, with large amounts of organic matter available for cave organisms, supporting rich aquatic and terrestrial communities (e.g., Bichuette and Trajano 2003, Simões et al. 2013, Bichuette et al. 2015).It is the second most intensively surveyed Brazilian karst area after the Alto Ribeira, but so far no comprehensive study on the bat communities from these caves has been undertaken.

Study Area
Passa Três cave is located in the Parque Estadual Terra Ronca -PETeR, one of the largest protected areas in Goiás state (Figures 1, 2).The Cerrado phytophysionomy, which is the second largest in Brazil, after Amazonian rainforest, comprises about 2 million km 2 (IBGE 2004).There is still significant economic activity represented by cattle rising and agriculture threatening the original Cerrado.Therefore, although the cave is located inside a Conservation Unit, its protection is not warranted.
The Passa Três cave (GO 14) (Lat -13,60 and Long -46,39), São Domingos County, is a relatively small and confined cave when compared with the cave systems in São Domingos karst area.The cave entrance (sinkhole -Figure 2A), used by the bats for emergence, is about 2.5 m high and 4 m wide; after a flooded low conduit, the cave stream joins the São Vicente I Cave.There are ca.1.5 km of vadose galleries potential for bat use, mostly the stream conduit, width and height varying from 2 to 8 m, with plenty of concavities and crevices in the ceiling and potential shelters.Stable spelaeoclimate due to spatial confinement and low noise in comparison with larger caves, make Passa Três cave a particularly good shelter.
According to Köeppen classification (1948), the climate is Aw type, with two welldefined seasons: dry (May-September) and wet (October-April), with annual precipitation between 600 and 2,000 mm (Lima and Silva 2005).

Sampling
Bats were sampled in three occasions at the Passa Três cave entrance; all in the dry season of 2000 (July 25, September 9 and 14), using one mist net (7× 3 m) at the beginning of the night (4 h total collecting efforts).Bats were euthanized through cervical dislodgment (one specimen or a couple per species) and identified using taxonomic keys (Vizotto andTaddei 1973, Gardner 2008).Vouchers of part of studied (collected) material are deposited in the Vertebrates Collection of Laboratório de Estudos Subterrâneos of Universidade Federal de São Carlos (LESV).

Results
An updated list of bats for Distrito Federal and Goiás state is shown in Table 1, with 66 species belonging to eight families, including 41 phyllostomids.Among these, 30 species were recorded in caves previously (22 belonging to Phyllostomidae family-see literature cited in Table 1).Two of them are threatened and included in the Brazilian List of Threat-  ened Fauna (MMA 2016): Lonchophylla dekeyseri (Figure 3), Endangered (EN), and Lonchorhina aurita (Figure 4), Vulnerable (VU) and the rare species, Lionycteris spurrelli.

Discussion
Majority of records for Distrito Federal (DF) and Goiás state (GO) are Phyllostomids, as observed by other authors (e.g.Bredt et al. 1999, Bezerra and Marinho-Filho 2010, Carrijo et al. 2011).In a regional scale, more than 40% of the species recorded in DF and GO use caves, which represents about 16% of Brazilian bats (30 of 179), a high percentage compared to other temperate regions.
Protection of roosting sites is an essential component of any strategy for the conservation of bats, with caves being the main roots for several bat species (Arita 1996, Kunz 1982).On the other hand, bat guano is an important food source for many subterranean organisms, especially for species restricted to subterranean habitats (troglobites), totally dependent on the resources present in these habitats and prone to rapid extinction following any ecological disequilibrium (e.g.alterations in the energy input), even very localized (Trajano 1995).Therefore, protecting bats is a fundamental part of any program or action for conservation of subterranean systems.
Passa Três cave follows the criteria of Site of Importance for Conservation of Bats (SICOM) according to the RELCOM.However, the proposition must be sent to RELCOM coordination for appreciation and validation.The following attributes were observed: presence of two threatened species -Lonchophylla dekeyseri and Lonchorhina aurita; presence of a rare species -Lionycteris spurrelli; a high diversity of bats compared to other caves in Brazil; reproducing colonies of at least three among these species, including the vulnerable L. aurita.Its protection is a priority since the Parque Estadual de Terra Ronca -PETeR has no Management Plan and is impacted by pastures, agriculture and poorly controlled visitation.In addition, the cave is the type-locality of two troglobitic (restricted to subterranean habitats) fishes, both included in the Brazilian Red List of Threatened species, the catfishes Ancistrus cryptophthalmus Reis, 1987 andItuglanis passensis Fernández andBichuette 2002 (Teleostei: Siluriformes) (Bichuette and Trajano 2003).Considering its peculiarity and attributes, it is urgent the inclusion of Passa Três in conservation programs, and implementation of monitoring programs.