Nippenose Valley, showing the location of Eiswert #1 cave and Lochabar Spring, source of Antes Creek (blue). Despite being only 445 m from each other, cave and surface sculpin populations are morphologically and genetically distinct. Notice that both cave and spring are very close by to a limestone quarry, which has the potential of being a conservation threat to the Pennsylvania grotto sculpin if further developed. Inset of Pennsylvania showing location of cave. Map data 2020 Google.