We conducted eDNA and visual surveys for cavefishes and cave crayfishes at 40 caves, wells, and springs across the Ozarks Highlands ecoregion. The estimated ranges of the cave crayfishes (i.e., Cambarus aculabrum, C. setosus, C. subterraneus, C. tartarus, Orconectes stygocaneyi) are outlined using United States Geological Survey 12-digit watersheds that encompass locations where these species have been observed. Troglichthys rosae is thought to restricted to the Springfield Plateau (light-grey outline); however, we detected the species at some of the sites enclosed by the circle. Typhlichthys eigenmanni was only surveyed at our two northern-most sites.