Style #1 for doing lens ablations A with the micromanipulator, slowly bring down the needle by the side of the lens and pressure down until it makes a perforation on the surface ectoderm/cornea B repeat these punctures around the lens C insert the needle between punctures, and with a coordinated motion of the hand holding the petri dish and the micromanipulator, gently pull the needle out to tear the tissue between punctures D put the needle on one side, under the lens and push it out of the optic cup.

  Part of: Espinasa L, Pavie M, Rétaux S (2023) Protocol for lens removal in embryonic fish and its application on the developmental effects of eye regression. Subterranean Biology 45: 39-52.