Style #3 for doing lens ablations A with the micromanipulator, slowly bring down the needle just by the side of the lens, closer than the previous two styles, and puncture the surface ectoderm. While inside the optic cup, position the needle so that it is above the lens instead of below. Gently scrape the overlaying agarose and scrape the tissue over the lens B the tissue will tear, and the lens will float up if there is liquid. It may need some nudging with the needle.

  Part of: Espinasa L, Pavie M, Rétaux S (2023) Protocol for lens removal in embryonic fish and its application on the developmental effects of eye regression. Subterranean Biology 45: 39-52.